irak4 rabbit polyclonal antibody  (Cell Signaling Technology Inc)

Journal: Cell Reports Medicine

Article Title: Osteoclast-derived apoptotic bodies inhibit naive CD8 + T cell activation via Siglec15, promoting breast cancer secondary metastasis

doi: 10.1016/j.xcrm.2023.101165

Figure Lengend Snippet: Siglec15 inhibits naive CD8 + T cell activation via blocking TLR2 co-stimulatory signaling pathways (A) Representative flow cytometry results of CD8 + T cell (stained with CFSE) exposed to different treatments. (B) Quantification of CD8 + T cell division and immune secretion (IFN-γ, TNF-α, IL-2; per well of 96-well plate), n = 3. (C) Western blot result showed expression of IRAK4, TRAF6, and TAK1 in CD8 + T cell lysate after different treatments, and quantification of the relative expression of TRAF6 in CD8 + T cells under different treatments, n = 3. (D) Representative IHC for IRAK4, TRAF6, and TAK1 in bone metastases sections from three groups of mice. Scale bar: 200 μm. (E) Semi-quantitative analysis of IRAK4, TRAF6, and TAK1 IHC staining images, n = 5. The data in each panel represent the means ± SD and were analyzed by Student’s two-tailed unpaired t test, ∗p < 0.05, ∗∗p < 0.01.

Article Snippet: Rabbit anti-IRAK4 , Bioss , Cat#bs-2440R;RRID: AB_11067218.

Techniques: Activation Assay, Blocking Assay, Flow Cytometry, Staining, Western Blot, Expressing, Immunohistochemistry, Two Tailed Test

Journal: Cell Reports Medicine

Article Title: Osteoclast-derived apoptotic bodies inhibit naive CD8 + T cell activation via Siglec15, promoting breast cancer secondary metastasis

doi: 10.1016/j.xcrm.2023.101165

Figure Lengend Snippet:

Article Snippet: Rabbit anti-IRAK4 , Bioss , Cat#bs-2440R;RRID: AB_11067218.

Techniques: Virus, Recombinant, Plasmid Preparation, Staining, Cell Isolation, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Purification, Bicinchoninic Acid Protein Assay, Isolation, Software